Fig. 8

Effects of the tyrosine protein kinase activity inhibitor K252a on Trk/γ-enolase complex formation in SH-SY5Y cells. A, B Representative co-immunoprecipitation (top) and quantification (bottom; as relative amount of γ-enolase in the immunoprecipitate in treated cells vs. control cells at 24 h) of γ-enolase and Trk from SH-SY5Y cells treated with K252a (200 nM) for 2 h and 24 h. After treatments, cells were lysed and immunoprecipitated (IP) with either non-specific rabbit IgG or rabbit anti-pan-Trk antibodies (Trk). The immunoprecipitates (A) and lysates for immunoprecipitation (B) were analysed by Western blotting probed with a mouse anti-γ-enolase antibody. In case of lysates, β-actin was used as loading control. Data are means of two independent experiments (n = 2) (one-way ANOVA, Dunnett’s test, *P < 0.05)