Fig. 6

Effects of the Rap1 processing inhibitor GGTI-298 on γ-Eno peptide-mediated neurite outgrowth of SH-SY5Y cells. SH-SY5Y cells were pre-treated with 5 µM GGTI-298 for 1 h, followed by treatment with 100 nM γ-Eno peptide. A After 48 h of γ-Eno peptide treatment, neurite outgrowth was determined by counting neurites, where cells with neurites longer than the cell diameter were scored. Data are means ± SD of three independent experiments (n = 5), each performed in quadruplicate (one-way ANOVA, Tukey’s test, *P < 0.05). B After 2 h of γ-Eno peptide treatment, F-actin content was determined by flow cytometry following staining with 500 ng/mL TRITC-conjugated phalloidin. Data are means ± SD of three independent experiments (n = 3), each performed in duplicate (one-way ANOVA, Tukey’s test, *P < 0.05). C Flow cytometry analysis of After 24 h of γ-Eno peptide treatment, MAP2 expression was determined by immunostaining with a specific MAP2 antibody. Data are means ± SD of three independent experiments (n = 3), each performed in duplicate (one-way ANOVA, Tukey’s test, *P < 0.05)