Fig. 5

Effects of the CPZ on γ-enolase-mediated Trk endosomal localisation in SH-SY5Y cells. Representative images (left) and quantification (right) as relative co-localisation area of Rab7 and Trk (solid arrows) of double immunofluorescence staining for the marker for late endosomes Rab7 (green fluorescence) and Trk (red fluorescence) in SH-SY5Y cells pretreated with 1 µM CPZ for 2 h, followed by treatment with 100 nM γ-Eno peptide for an additional 30 min, in serum-free medium. Data are means ± SD of the pixels in the third quadrant of the scatter plot (cell numbers ≥ 10) (one-way ANOVA, Tukey’s test, * P ˂ 0.05). Scale bars: 10 µm