Fig. 4

Effects of γ-Eno peptide on Trk localisation in clathrin-coated vesicles in SH-SY5Y cells. Representative images (left) and quantification (right) as relative co-localisation area of Trk and clathrin (solid arrows) of double immunofluorescence staining for Trk (green fluorescence) and clathrin heavy chain (red fluorescence) in SH-SY5Y cells pretreated with 1 µM CPZ for 2 h, followed by treatment with 100 nM γ-Eno peptide for an additional 30 min in serum-free medium. Data are means ± SD of the pixels in the third quadrant of the scatter plot (cell numbers ≥ 10) (one-way ANOVA, Tukey’s test, * P ˂ 0.05). Scale bars: 10 µm