Fig. 3

Effects of γ-Eno peptide on Trk cell-surface expression of SH-SY5Y cells. A Quantification from flow cytometric analysis of non-permeabilised cells for cell-surface expression of Trk as a time course of SH-SY5Y cells treated with 100 nM γ-Eno peptide in the absence and presence of 1 µM CPZ in serum-free medium. Data are means ± SD of three independent experiments (n = 3), each performed in duplicate (one-way ANOVA, Tukey’s test, *P < 0.05). B Representative images (left) and quantification (right) as relative co-localisation area of cadherin and Trk (solid arrows) of double immunofluorescence staining for the plasma membrane marker cadherin (green fluorescence) and Trk (red fluorescence) in SH-SY5Y cells pretreated with 1 µM CPZ for 30 min, followed by treatment with 100 nM γ-Eno peptide for an additional 30 min in serum-free medium. Data are means ± SD of the pixels in the third quadrant of the scatter plot (cell numbers ≥ 10) (one-way ANOVA, Tukey’s test, * P ˂ 0.05). Scale bars: 10 µm