Fig. 2

γ-Enolase localisation in clathrin-coated vesicles in SH-SY5Y cells. Representative images (left) and quantification (right; as relative co-localisation area of clathrin and γ-enolase) of double immunofluorescence staining for clathrin heavy chain (green fluorescence) and γ-enolase (red fluorescence) in wild-type and transfected SH-SY5Y cells with down-regulated γ1-syntrophin expression (Stealth RNAi SNTG1). Stronger co-localization of γ-enolase with clathrin heavy chain (solid arrows) was observed in wild type cells compared to transfected cells. Data are means ± SD of the pixels in the third quadrant of the scatter plot (cell numbers ≥ 10) (two tailed t test, * P ˂ 0.05). Scale bars: 10 µm