Table 2 Summary of the cell-type-specific differences involving bioenergetics, relative expression of SDHs, SDHs-multiprotein assembly, and PTMs of SDHs observed under CAMKK2 deletion conditions in HEK293 and HepG2 cells
Cell line | HEK293 | HepG2 |
|---|---|---|
Effect of CAMKK2 deletion | ||
Tissue source | Embryonic kidney | Liver |
Origin/disease | Transformed cell line | Hepatocellular carcinoma |
Morphology | Epithelial | Epithelial-like |
Basal cellular respiration | Decreased | Decreased |
CI-IV respiration | Increased | Decreased |
CII-IV respiration | Increased | Decreased |
SDHA mRNA level | Decreased | Unaltered |
SDHB mRNA level | Increased | Decreased |
SDHC mRNA level | Unaltered | Unaltered |
SDHA protein level | Increased | Unaltered |
SDHB protein level | Increased | Decreased |
SDHC protein level | Increased | Unaltered |
SDHB-associated MPC | Shifted to the higher mol. weight | Shifted to the lower mol. weight |
SDHB PTM state | Charged fractions altered | Charged fractions unaltered |
SDH enzymatic activity | Increased | Decreased |