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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: CAMKK2 regulates mitochondrial function by controlling succinate dehydrogenase expression, post-translational modification, megacomplex assembly, and activity in a cell-type-specific manner

Fig. 8

Knockdown and overexpression of SDHB in CAMKK2−/− HEK293 and HepG2 cells, respectively. A, C Immunoblots showing ATP5A (A), GAPDH (B), and SDHB (A, B) protein levels in the SDHB knockdown (A) and SDHB overexpressed ER/mitochondrial fractions derived from CAMKK2−/− HEK293 and HepG2 cells, respectively. B, D Bar graphs showing the relative amount of SDHB protein in the SDHB knockdown (B) and SDHB overexpressed ER/mitochondrial fractions derived from CAMKK2−/− HEK293 and HepG2 cells, respectively. N = 6 replicates from 2 independent experiments. Data represented as Mean ± SEM. Statistical analysis by t-test (unpaired). E, F SDH enzymatic activity in the enriched ER/mitochondrial fractions derived from parental, CAMKK2−/−, and CAMKK2−/− + SDHB knockdown/overexpressed HEK293 (E) and HepG2 (F) cells, respectively. N = 10 replicates from 2 independent experiments. Statistical significance from one-way ANOVA followed by multiple comparisons

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Cell Communication and Signaling

ISSN: 1478-811X