Skip to main content
Fig. 4 | Cell Communication and Signaling

Fig. 4

From: CAMKK2 regulates mitochondrial function by controlling succinate dehydrogenase expression, post-translational modification, megacomplex assembly, and activity in a cell-type-specific manner

Fig. 4

Effect of CAMKK2-deficiency on the abundance of OXPHOS-associated proteins in HEK293, EA.hy926, and HepG2 cell-derived enriched ER/mitochondrial fractions. A, B Immunoblots showing abundance of OXPHOS-associated proteins. The red rectangle marked areas indicate a relatively high level of SDHB in CAMKK2−/− HEK293 cell clones (clone-A5 and -A10) compared to the parental cells. The blue rectangle marked area in B indicates a relatively low level of SDHB in CAMKK2−/− HepG2 cell clone (clone-C1) compared to the parental cells. Yellow arrows indicate longer exposure of some segment of the top immunoblot to highlight bands that are underexposed. C, D Scatter plots showing relative level of SDHB in different CAMKK2 deleted cell clones. Statistical significance by one-way ANOVA followed by multiple comparisons (EG). “×” indicates fold change

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X