Fig. 2

Glioma organoids. a Obtain glioma organoids through gene editing. CRISPR/Cas9 mutagenesis and Sleeping Beauty (SB) transposon-mediated gene insertion have served for this purpose by introducing clinically relevant oncogenic mutations into healthy human cerebral organoids in order to develop glioma tumors. b Glioma organoids were obtained by co-cultivation with GSCs. Cerebral organoids were obtained from 3D human embryonic stem cells or patient iPSCs, and then co-cultured with patient-derived GSCs. c Tumor derived glioma organoids. Cut the excised glioma tissue into pieces with a diameter of about 1 mm. The tumor pieces were cultured in the GBO medium on an orbital shaker for 1–2 weeks. Created with BioRender.com