Fig. 6

ADQ inhibits breast cancer immune escape and lung metastasis in vivo by suppressing the TAM/CXCL1/Treg pathway. a–c ADQ inhibits mammary tumor growth by suppressing TAM/CXCL1 activity. TAM/rCXCL1 refers to the overexpression of CXCL1 in the co-injected TAMs, while TAM/shCXCL1 refers to the knockdown of CXCL1 in the co-injected TAMs. a The representative pictures of tumors in each group. N = 6. b Tumor weights of each group. N = 10. c Tumor volume curves of each group. N = 10. d Both the in vivo imaging assay and lung HE staining assay suggested that ADQ dramatically suppressed the lung metastasis of mammary tumors by inhibiting the TAM/CXCL1 pathway. Scale bar = 100 μm. N = 3. Arrows and circles indicate the metastatic tumor foci in murine lungs. e–f TIL infiltration level (e) and tissue apoptosis (f) in mammary tumors were detected by HE staining assay and immunofluorescence assay, respectively. Scale bars indicate 100 μm in € and 20 μm in (f). N = 3. g The infiltration levels of Tregs, naive CD4⁺ T cells, and TAMs in mammary tumors of each group were detected by flow cytometry. N = 3. *p < 0.05; **p < 0.01