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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Ceramide from sphingomyelin hydrolysis induces neuronal differentiation, whereas de novo ceramide synthesis and sphingomyelin hydrolysis initiate apoptosis after NGF withdrawal in PC12 Cells

Fig. 6

The effect of specific ceramide inhibition on PC12 or TrkA cells following NGF removal. Differentiated PC12 and TrkA cells were deprived of NGF for 24 h in the presence of either 15 μM desipramine or 25 μM FB1. Control cells treated with vehicle or inhibitors alone are in white, cells deprived of NGF are depicted with hatching. Each value represents the mean of duplicate determinations from three independent experiments; bars, SEM; *p < 0.05, **p << 0.05, ***p << 0.01. A Measurement of ceramide levels. Lipids were extracted and quantified by DAG kinase assay as described in Materials and Methods. B Measurement of SM levels. Lipids were extracted and quantified by SM mass assay as described in Materials and Methods. C Measurement of viability. A minimum of 250 cells was scored by trypan blue exclusion for the incidence of apoptosis in each experiment

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Cell Communication and Signaling

ISSN: 1478-811X