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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Ceramide from sphingomyelin hydrolysis induces neuronal differentiation, whereas de novo ceramide synthesis and sphingomyelin hydrolysis initiate apoptosis after NGF withdrawal in PC12 Cells

Fig. 5

Response to NGF withdrawal in PC12 or TrkA cells. Differentiated PC12 (UPPER) and Trk A (LOWER) cells were deprived of NGF for up to 48 h and collected every 3–6 h for viability assay as well as extraction and quantification of lipids. Quantification of apoptosis (viability) was performed by trypan blue staining of dead cells as well as by staining the cells with the DNA specific fluorochrome Hoechst 33,258 as described in Materials and Methods. A minimum of 250 cells was scored for the incidence of apoptosis in each experiment. Endogenous ceramide was quantified by DAG kinase assay and sphingomyelin by SM mass assay as described in Materials and Methods. Each value represents the mean of duplicate determinations from at least three independent experiments. bars, SEM

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Cell Communication and Signaling

ISSN: 1478-811X