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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: Ceramide from sphingomyelin hydrolysis induces neuronal differentiation, whereas de novo ceramide synthesis and sphingomyelin hydrolysis initiate apoptosis after NGF withdrawal in PC12 Cells

Fig. 4

The effect of desipramine on differentiation of PC12 and TrkA cells. A Time course for ceramide generation in PC12 cells treated as above. Lipids were extracted and ceramide levels quantified by the DG kinase assay as described in Materials and Methods. B Time course for SM generation in PC12 cells treated as above. Lipids were extracted and SM levels quantified by the SM mass assay as described in Materials and Methods. C Time course for ceramide generation in TrkA cells treated as above. Lipids were extracted and ceramide levels quantified by the DG kinase assay as described in Materials and Methods. D Time course for SM generation in TrkA cells treated as above. Lipids were extracted and SM levels quantified by the SM mass assay as described in Materials and Methods. Each value represents the mean of duplicate determinations from at least three experiments; bars, SEM; **p ≤ 0.01

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Cell Communication and Signaling

ISSN: 1478-811X