Fig. 5
From: Pharmacologic targeting of the P-TEFb complex as a therapeutic strategy for chronic myeloid leukemia
Wogonin modulated P-TEFb kinase activity in vivo. a The protein expression of p-RNA Pol II (Ser2), p-RNA Pol II (Ser5), CyclinT1, CDK9 and MCL-1 in KU-812 xenografts. β-actin was used as a loading control. The results are representative of three independent experiments (mean ± SD). *p < 0.05, **p < 0.01, compared with control group. b Immunofluorescence and stained with p-RNA Pol II (Ser2) (green fluorescence), p-RNA Pol II (Ser5) (green fluorescence) and MCL-1 (green fluorescence) respectively, as well as DAPI (blue fluorescence) in tumor of KU-812-bearing NOD/SCID mice. They were detected by confocal microscopy (FV1000; Olympus) with FV10-ASW2.1 acquisition software (Olympus) at room temperature (Original magnification × 600). c The expression of CD71 and GPA was detected in tumor of KU-812-bearing NOD/SCID mice by flow cytometry analyses. d Quantification of the expression of CD71/GPA. Data represent mean ± SD of three independent experiments. ***p < 0.001, compared with control group