Fig. 3
From: Hhex inhibits cell migration via regulating RHOA/CDC42-CFL1 axis in human lung cancer cells
Hhex interacted with RHOA and CDC42. a 293FT cells were co-transfected with plasmids of pcDNA3.1 and pcDNA3.1-HHEX-HA or plasmids of pcDNA3.1-HHEX-HA and pcDNA3.1-RHOA-MYC, cell lysate was immunoprecipitated with anti-HA antibody, Hhex and RHOA were detected by western blot. The images are representative of three independent experiments with similar results. b 293FT cells were co-transfected with plasmids of pcDNA3.1 and pcDNA3.1-RHOA-MYC or plasmids of pcDNA3.1-HHEX-HA and pcDNA3.1-RHOA-MYC, cell lysate was immunoprecipitated with anti-MYC antibody, Hhex and RHOA were detected by western blot. The images are representative of three independent experiments with similar results. c 293FT cells were co-transfected with plasmids of pcDNA3.1 and pcDNA3.1-HHEX-HA or plasmids of pcDNA3.1-HHEX-HA and pcDNA3.1-CDC42-MYC, cell lysate was immunoprecipitated with anti-HA antibody, Hhex and CDC42 were detected by western blot. The images are representative of three independent experiments with similar results. d 293FT cells were co-transfected with plasmids of pcDNA3.1 and pcDNA3.1-CDC42-MYC or plasmids of pcDNA3.1-HHEX-HA and pcDNA3.1-RHOA-MYC, cell lysate was immunoprecipitated with anti-MYC antibody, Hhex and CDC42 were detected by western blot. The images are representative of three independent experiments with similar results