Fig. 1

Glioblastoma-derived EVs promote the proliferation of glioblastoma cells. A. U87-EVs-treated U87 cells exhibited higher proportion of EdU positive cells than DMSO-treated cells in the EdU incorporation assay. GW4869 lowered the proportions of EdU positive signal in U87 cells. The addition of U87-derived EVs partially reversed the GW4869-reduced EdU positive signals. U87-EVs did not alter the proportions of Ki67 positive cells. GW4869 reduced the proportions of Ki67 positive cells, and the addition of U87-EVs partially reversed this reduction. B. A172-EVs-treated A172 cells exhibited higher proportion of EdU positive cells than DMSO-treated cells in the EdU incorporation assay. GW4869 lowered the proportions of EdU positive signal in A172 cells. The addition of A172-derived EVs partially reversed the GW4869-reduced EdU positive signals. A172-EVs did not alter the proportions of Ki67 positive cells. GW4869 reduced the proportions of Ki67 positive cells, and the addition of A172-EVs partially reversed this reduction. C. LN229-EVs-treated LN229 cells exhibited higher proportion of EdU positive cells than DMSO-treated cells in the EdU incorporation assay. GW4869 lowered the proportions of EdU positive signal in LN229 cells. The addition of LN229-derived EVs partially reversed the GW4869-reduced EdU positive signals. LN229-EVs did not alter the proportions of Ki67 positive cells. GW4869 reduced the proportions of Ki67 positive cells, and the addition of LN229-EVs partially reversed this reduction. D. U87-EVs-treated U87 cells exhibited higher CCK8 signals. GW4869 largely lowered CCK8 signals and U87-EVs significantly reversed the CCK8 signal reduced by GW4869. A172-EVs-treated A172 cells exhibited higher CCK8 signals. GW4869 largely lowered CCK8 signals and A172-EVs significantly reversed the CCK8 signal reduced by GW4869. LN229-EVs did not alter CCK8 signals in LN229 cells. GW4869 largely lowered CCK8 signals and U87-EVs significantly reversed the CCK8 signal reduced by GW4869. Results are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Scale bar, 100 μm