Fig. 1

Detection of ecDHFR DD-fused mCherry expression induced by TMP. a Schematic representation of the engineered ecDHFR DD-fused mCherry expression plasmids. ecDHFR DD was fused to the C- or N-terminal of mCherry. b Illustration of the mechanism by which conditionally destabilized ecDHFR DD regulates mCherry expression. mCherry gene is fused with the ecDHFR DD, which triggered its rapid degradation. The TMP prevents the destabilization and degradation of mCherry. c Red optical density statistics of mCherry expression in mCherry-, mCherry-ecDHFR DD-, and ecDHFR DD-mCherry-transfected 293T cells in the presence of TMP (0, 1, 2, 4, 6, or 8 ng/μL) for 24 h. mCherry integrated density was detected at 24 h post-TMP removal