Table 1 Antibodies and inhibitors employed in this study
From: PI3K-AKT, JAK2-STAT3 pathways and cell–cell contact regulate maspin subcellular localization
Brand | Antibody/inhibitor | Experiment | Dilution/dose |
|---|---|---|---|
Millipore (MABC603) | Mouse anti-maspin | WB | 1:1000 |
Sigma (HPA019132) | Rabbit anti-maspin | IF | 1:100 |
Sigma (HPA019125) | Rabbit anti-maspin | IF | 1:100 |
Santa Cruz Biotech (373745) | Mouse anti-EGFR(A-10) | WB; IP | 1:1000; 1:50 |
Cell Signaling (#14472) | Mouse anti-E-cadherin | IF | 1:100 |
Santa Cruz Biotech (5286) | Mouse α-tubulin (clone b7) | WB | 1:1000 |
Santa Cruz Biotech. (47778) | Mouse anti-β-actin | WB | 1:1000 |
Thermo Scientific (mab636) | Mouse anti-lamin A/C | WB | 1:1000 |
Cell Signaling (9275S) | Rabbit anti-p-Akt (T308) | WB | 1:1000 |
Santa Cruz Biotech. (81434) | Mouse anti-Akt1/2/3 | WB | 1:1000 |
Sigma | anti-rabbit HRP | WB | 1:10,000 |
Sigma | anti-mouse HRP | WB | 1:10,000 |
Invitrogen | anti-rabbit Alexa Fluor 488 | IF | 1:500 |
Invitrogen | anti-mouse Alexa Fluor 594 | IF | 1:500 |
AdooQ Biosciences (#A10422) | Gefitinib (Iressa) | EGFR | 10 µM |
AdooQ Biosciences (#A11161) | Wortmannin | PI3K | 10 µM |
AdooQ Biosciences (#A11795) | WP1066 | JAK2/STAT3 | 10 µM |
AdooQ Biosciences (#A12723) | PP2 | Src | 10 µM |
AdooQ Biosciences (#A12368) | FTI-277 | H/K-Ras | 10 µM |
AdooQ Biosciences (#A13790) | Go6983 | PKC | 10 µM |
AdooQ Biosciences (#A11148) | BX-795 | PDK1 | 5 µM |
AdooQ Biosciences (#A10782) | Rapamycin (Sirolimus) | mTOR | 10 µM |
AdooQ Biosciences (#A15279) | VO-OHpic | PTEN | 10 µM |
AdooQ Biosciences (#A13210) | Triciribine | Akt | 10 µM |