Fig. 5
From: PI3K-AKT, JAK2-STAT3 pathways and cell–cell contact regulate maspin subcellular localization
Cell–cell contact regulates maspin nuclear translocation in MCF-10A cells. A MCF-10A cells were plated at high confluency. The next day, cells were starved for 24 h followed by 20 ng/ml EGF treatment for the indicated intervals. Maspin distribution was analyzed by immunofluorescence B MCF-10A cells were treated with 4 mM of EGTA for 30 min. Maspin and E-cadherin patterns were examined by immunofluorescence. C Cells were quantified based on the criteria shown next to the graph *p < 0.05 (Chi-square test). D MCF-10A cells were plated at the indicated cell densities and maspin localization was analyzed by immunofluorescence. E Cells were quantified based on the criteria shown next to the graph, *p < 0.05 (Chi-square test). Images are representative of at least three independent assays. Scale bar: 20 μm