Fig. 3
From: PI3K-AKT, JAK2-STAT3 pathways and cell–cell contact regulate maspin subcellular localization
PI3K and Akt, but not PDK1, regulate maspin nuclear accumulation. A Starved MCF-10A cells were pretreated with inhibitors of the indicated signaling molecules (except for the three panels on the upper set, left column) for 30 min followed by EGF treatment (20 ng/mL) for additional 1 h. Maspin localization was analyzed by immunofluorescence with anti-maspin antibody. Nuclei were stained with DAPI. B Cells were quantified based on the criteria shown next to the graph *p < 0.05 (Chi-square test). C Starved MCF-10A cells were treated as described in A. Cell lysates were analyzed by immunoblot with anti-phospho-Akt (T308) antibody (upper panel, arrow). The membrane was reprobed with anti-Akt (lower panel). MW markers are indicated on the left side of the image. The pAkt and total Akt bands were quantified and pAkt/Akt ratios were plotted on a graph. Images are representative of at least three independent assays. Scale bar: 20 μm