Fig. 2
From: PI3K-AKT, JAK2-STAT3 pathways and cell–cell contact regulate maspin subcellular localization
EGF-induced maspin nuclear accumulation depends on the PI3K-Akt and JAK2-STAT3 pathways A Starved MCF-10A cells were pretreated with inhibitors of EGFR (Gefitinib), PI3K (Wortmannin), pan-PKC (Go6983), JAK2/STAT3 (WP1066), H/K-Ras (FTI-277) and Src family kinases (PP2) (except for the upper row), for 30 min followed by 20 ng/ml EGF treatment for additional 1 h. Maspin localization was analyzed by immunofluorescence with anti-maspin antibody. Nuclei were stained with DAPI. B Cells were quantified based on the criteria shown next to the graph *p < 0.05 (Chi-square test). C The experiment described in A was repeated and images were acquired in a higher resolution. JAK2/STAT3 inhibition by WP1066 leads to a junctional discontinuity pattern, whereas PI3K inhibition by Wortmannin results in continuous maspin distribution across cell–cell junctions (arrows). Images are representative of at least three independent assays. Scale bar: 20 µm