Fig. 8

The key communications between macrophage (M) and T cell subpopulation. a The dot plot showing the key ligands in the outgoing signaling pattern of subpopulation as secreting cells. b Circle plot showing the inferred intercellular communication network for CXCL signaling. Arrows and edge color indicate direction ((source: target). Edge thickness indicates the sum of weight key signals between populations. c Hierarchical network diagram of significantly inferred intercellular communication network for IGF signaling based on above-mentioned cell-cell communication pattern (from macrophage to T cell). d Heatmap shows the relative importance of each cell subtype based on the computed four network centrality measures of CXCL signaling (upper) and relative contribution of each ligand–receptor pair to the overall communication network of CXCL signaling. e Heatmap shows the relative importance of each cell subtype based on the computed four network centrality measures of IGF signaling and the relative contribution of each ligand–receptor pair to the overall communication network of IGF signaling. f Significant ligand–receptor pairs involving CXCL and IGF pathway sending signals from TREM2^high M to four T cell states. g FACS dot plots showing the expression of PD-1 on CD8⁺ T cells. h The percentages of PD-1⁺ in CD8⁺ T cells (the first panel). The serum levels of IL6, IL1β, IL-10, TNF-α, CXCL10, GALECTIN-1, and IGF1 in different groups are shown in boxplot (mean ± SD). Statistical analysis by Student’s t-test