Fig. 4

ARF19 condensates are initially liquid-like and lack higher-order oligomers. A Time lapse images of wild-type ARF19 condensates in protoplasts. The top images are from the beginning of a time-lapse series started shortly after protoplast transfection (early ARF19 condensates) and show an example where the condensates have liquid-like behavior. The bottom images are from a later time point in a time lapse series (late ARF19 condensates) and show an example where the condensates appear to partially fuse but ultimately are unable to fully fuse resulting in a ‘grape-bunch’ like morphology. Note, time intervals between the bottom panels are not equal from panel to panel. B Each point shows the percent values for either lower order oligomers (left) or higher order oligomers (right) for individual ARF19 condensates approximately two or sixteen hours after protoplast transfection N = 16 (3 h) and N = 17 (16 h). C Table summarizing the data from figure panel B