Table 3 CRC HAE culture cell yield and success rate depending on the tissue collected
From: Long-term differentiating primary human airway epithelial cell cultures: how far are we?
Sampling method | Age group (condition) | Initial cell number | Subculturing method | Success rate in propagation | References |
|---|---|---|---|---|---|
Induced sputum | Children |  < 2000 cells/ portion | CRC HAE mono (Rho/SMAD inhibition) | 20% in CF patients | Mou et al. [71] |
Tracheal aspirate | Neonatal |  < 100 cells/ aspirate | CRC HAE mono (Rho/SMAD/mTOR inhibition) | 40% from single sample, 80% if multiple samples/ patient | Lu et al. [74] |
BAL | Neonatal |  < 2000 cells/ portion | CRC HAE, mono (Rho/SMAD inhibition) | 100% (multiple samples/ patient) | Mou et al. [71] |
Nasal brushing | N/A (CF or healthy) | 0.4 to 1.5 million viable cells | Traditional ALI culture | Culture initiation: CF patients—66%; healthy—85% Differentiation in ALI: 100% | Schogler et al. [78] |
Bronchial brushing | N/A | 0.045 – 0.2 mln cells/ brush | Indirect CRC HAE co-culture: HAE cells cultured with conditioned fibroblast medium with ROCKi | N/A | Wolf et al. [65] |
Bronchial brushing (non-bronchoscopic) | Children (healthy or asthmatic) |  ~ 2,67 mln cells/ 2 brush passes | CRC HAE co-culture, direct | N/A | Martinovich et al. [50] |