Fig. 2
From: Mitochondrial DNA leakage induces odontoblast inflammation via the cGAS-STING pathway
The cGAS-STING pathway was activated by LPS in mDPC6T cells. a–b The expression of cGAS and STING and the phosphorylation of TBK1 in mDPC6T cells were assessed by western blotting. mDPC6T cells were treated with different concentrations of LPS (1, 5, 10, 15, and 20 μg/ml) for 24 h (a) or 20 μg/ml LPS for 0, 6, 12, and 24 h to induce inflammation (b). The quantitative data represent the relative ratio of the target protein to total TBK1 or GAPDH. c Real-time PCR was performed to quantify the relative mRNA levels of cGAS and STING in mDPC6T cells after 24 h of stimulation with different concentrations of LPS. The data are the mean ± SEM, n = 3 (*p < 0.05, **p < 0.01 ***p < 0.001)