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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation

Fig. 8

Effect of CMA blockage on downstream UPR and apoptosis pathway in a xenograft mouse model. a UPR activation was examined by protein expression of p-PERK, p-IRE1, p-JNK and CHOP. Significant up-regulation of p-PERK, p-IRE1, p-JNK and CHOP was observed in the LAMP-2A shRNA (LAMP-2A shR) group as compared with control shRNA (CT-shR) group. b Activator proteins in apoptosis including caspase 3, caspase 9 and Bax, and anti-apoptotic protein Bcl-2 were measured by western blot. Caspase 3, caspase 9 and Bax expressions were enhanced while Bcl-2 expression was inhibited in LAMP-2A shR group as compared with CT-shR group. c TUNEL results of differently treated groups. Significantly more apoptotic cells were found in LAMP-2A shR group as compared with CT-shR group. The data are mean ± SEM from three mice as a group. The levels of p-PERK, p-IRE1 and p-JNK are compared with levels of PERK, IRE1 and JNK respectively, while the rest of the proteins are compared with β-actin. Protein levels are expressed relative to vehicle group set as 1. Significant changes are set as p < 0.05 and represented by asterisk (One-Way ANOVA; Bonferroni's test)

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Cell Communication and Signaling

ISSN: 1478-811X