Fig. 5
From: Peptide targeting of lysophosphatidylinositol-sensing GPR55 for osteoclastogenesis tuning
LPI induces filopodium appearance through endogenous GPR55 in RAW264.7 cells. RAW264.7 cells interfered with non-targeting (si-NT) or Gpr55-specific (si-GPR55) siRNAs were serum deprived for 2 h, stimulated without or with 10 µM soybean LPI for 15 min, fixed and stained for confocal imaging. Phalloidin staining allows visualisation of filamentous actin, while Hoechst staining reveals cell nuclei (see “Materials and methods” section for details). a Representative confocal images of actin staining, as indicated. Scale bars, 5 µm. b Quantification of filopodium formation, as described in “Materials and methods” section. Data are expressed as proportions (%) of the unstimulated si-NT, as means (± SEM) of three independent experiments, each carried out in duplicate. *p < 0.05 versus LPI-stimulated si-NT (one-way ANOVA, followed by Tukey test)