Fig. 2

Representative immunohistochemical staining of PPL, EVPL, PLEC and CA125 on formalin fixed paraffin embedded (FFPE) serous ovarian benign, Type I (low grade) and Type II (high-grade) ovarian tumours. Immunohistochemistry images of FFPE sections representing staining of PPL, PLEC, EVPL and CA125 of benign, Type I (low-grade) and Type II (high-grade) ovarian tumours. Samples were obtained from patients diagnosed with ovarian cancer before surgery under protocols approved by the Human Research and Ethics Committee (Ethics approval #09/09) of the Royal Women’s Hospital, Melbourne, Australia after gaining patient's consent. Immunohistochemistry was performed as described previously [150, 155]. Sections were assessed microscopically for positive DAB (brown), haematoxylin (blue) counterstain staining. Magnification (40×), scale bar = 50um