Fig. 7

CD characterisation of CP12: high helical content and putative coiled coil arrangement. a CD spectra of 10 µM CP12 in 50 mM phosphate buffer pH 6.5 with increasing concentration of TFE. These spectra indicate a high proportion of helical structure, which increases further in the presence of TFE. b, c Result of the Paircoil predictor for the search of putative coiled coil motif for each T. pseudonana CP12 residues (b) and C. reinhardtii CP12 residues (c). The coiled coil prediction is shown as a score varying from 0 to 1 (a score of 0 indicates a high probability to be in a coiled coil region and in contrast a score of 1, a low probability). The threshold for a region to have a high probability to form a coiled coil structure is indicated with a dotted line (score < 0.025). The AWD_VEEL regions are highlighted in grey, and the position of the cysteine residues are indicated with a C