Fig. 4

Transcriptome-wide off-target determination with the dPspCas13b-RESCUE-NES and dRanCas13b-RESCUE-NES systems. a Heatmap of the A-to-I editing rate of adenines covered by dPspCas13b-RESCUE-NES and dRanCas13b-RESCUE-NES systems targeting KRAS, CTNNB1, and RAF1 genes. Each target contains two experimental repeats. b Heatmap of the C-to-U editing rate on cytosine covered by dPspCas13b-RESCUE-NES and dRanCas13b-RESCUE-NES system targeting at KRAS, CTNNB1, and NFKB1 gene. Each target contains two experiment repeats. c Manhattan plots of dPspCas13b-RESCUE-NES and dRanCas13b-RESCUE-NES A-to-I off-targets. n, total number of A-to-I off-target SNPs. d Manhattan plots of dPspCas13b-RESCUE-NES and dRanCas13b-RESCUE-NES C-to-U off-targets. n, total number of C-to-U off-target SNPs. e Comparison of A-to-I RNA off-target numbers between dPspCas13b-RESCUE-NES and dRanCas13b-RESCUE-NES systems. Bars represent the mean ± SEM. Different asterisks indicate significant differences as follows: **p < 0.01; ***p < 0.001. f The comparison of C-to-U RNA off-target numbers between dPspCas13b-RESCUE-NES and dRanCas13b-RESCUE-NES systems. Bars represent the mean ± SEM. Different asterisks indicate significant differences as follows: **p < 0.01; ***p < 0.001