Fig. 3

Substitution of IKKβ 177Ser/Gly mediated by dPspCas13b-RESCUE-NES system. a Heatmap of the A-to-I editing rate on adenosines covered by dPspCas13b-RESCUE-NES system targeting the IKKβ mRNA. Three gRNAs that target A and are positioned at 24, 25, and 26 were designed. Three replicates were performed per gRNA for A-to-I editing efficiency statistics. b The effective editing of targeted IKKβ mRNA contributed to IKKβ-related gene expression detection. Bars represent the mean ± SEM. Different asterisks indicate significant differences as follows: *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance. c The red optical density statistics of p65 proteins in the cell nucleus. Statistical method: The analysis tool Image J was used to determine the grey scale of the mCherry protein located in the nucleus of cells in the IKKβ-177 Ser gRNA group and the non-target control group for semiquantitative analysis. Three representative pictures from each group were analysed. Bars represent mean ± SEM. “**”p < 0.01