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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Hepatitis B virus X protein promotes vimentin expression via LIM and SH3 domain protein 1 to facilitate epithelial-mesenchymal transition and hepatocarcinogenesis

Fig. 5

The interaction of HBX with LASP1 and vimentin and HBX promotes vimentin stability through LASP1 in hepatoma cells. a The interaction of HBX with LASP1 and vimentin measured by Co-IP assays in hepatoma cells. b The prokaryotic expressions of HBX with GST Tag, LASP1 with GST Tag, vimentin with His Tag, LASP1 with His Tag detected by western blot. c The interaction of HBX with LASP1 and vimentin measured by GST-pull down assays. d The colocalizations of HBX, LASP1, and vimentin in hepatoma cells were detected immunofluorescence assay (× 400). e The schematic diagram of three HBX mutants, LASP1 mutants, and vimentin mutants. The domain and region distribution in LASP1 and vimentin protein were as the description in the UniProt database. f The protein expressions of HBX and associated mutants with HA Tag, LASP1 and associated mutants with Flag Tag, vimentin and associated mutants with HA Tag, in HEK293T cells detected by western blot. g The interaction of HBX and associated mutants with LASP1, vimentin and associated with mutant in 293 T cells detected by Co-IP assay. VIM vimentin

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Cell Communication and Signaling

ISSN: 1478-811X