Fig. 2

Modulation of caveolin 1 and p21^Waf1 expression in CAFs generated following intimate or distant coculture of normal human fibroblasts with breast cancer cells. a Western blot analyses of Cav-1 and p21^Waf1 in AG1522 CAFs that were cocultured for 120 h with MCF7, MDA-MB-231, or themselves using the 1 μm permeable microporous membrane inserts. The results indicate that protein expression is altered in the AG1522 CAFs depending upon coculture with MDA-MB-231 or MCF7. Staining with Ponceau S Red was used for loading control and to determine fold-change in expression level. b Microscopic images (63X oil objective on a Zeiss Axiovert 200 M) of in situ immunofluorescence (scale bar = 20 μm) of Cav-1 expression in AG1522 cells that had been in co-culture with AG1522 cells (control) (left), or with MDA-MB-231 (center) or MCF7 (right) breast cancer cells for 120 h