Fig. 6

Exogenous Wnt7a ligand treatment recovery dendritic arbor after inhibition of PORCN. Sister cultures of embryonic hippocampal neurons were used to quantify the recovery of dendritic arbor after treatment with different concentrations of the canonical Wnt7a ligand, and stained with MAP1B (red), ATF-2 (blue) and phalloidin (green). Neurons were imaged using a confocal microscope to evaluate neuronal morphology. A See representative micrographs of control neurons and treated with Wnt7a (50 ng/ml), 10 nM Wnt-C59 or plus Wnt7a (50, 100, 200 ng/ml) for 24 h, White arrowhead show actin on the tip of different neurites. B Graph shows quantification of average of neurites length (see left graph) and recovery of dendritic complexity (see right graph). The results are presented as the mean ± SEM for n = 5 experiments, **p < 0.01, ***p < 0.001. Bonferroni’s test. Bar represents 10 μm