Fig. 5
From: Morphological neurite changes induced by porcupine inhibition are rescued by Wnt ligands
Exogenous Wnt5a ligand treatment recovers dendritic arbor after inhibition of PORCN. Sister cultures of embryonic hippocampal neurons were used to quantify the recovery of dendritic arbor, after treatment with different concentrations of the non-canonical Wnt5a ligand. A See representative micrographs of control neurons and treated with Wnt5a (50 ng/ml), 10 nM Wnt-C59 or plus Wnt5a, (50, 100, 200 ng/ml) for 24 h, White arrowhead show actin on the tip of different neurites. B Graph shows quantification of average of neurite length (see left graph) and recovery of dendritic complexity (see right graph). The results are presented as the mean ± SEM for n = 5 experiments, **p < 0.01. Bonferroni’s test. Bar represents 10 μm