Fig. 4
From: Morphological neurite changes induced by porcupine inhibition are rescued by Wnt ligands
Exogenous Wnt3a ligand treatment recovery dendritic arbor after inhibition of PORCN. Sister cultures of embryonic hippocampal neurons were used to quantify the recovery of dendritic arbor after treatment with different concentrations of the canonical Wnt3a ligand. A See representative micrographs of control neurons and treated with Wnt3a (50 ng/ml), 10 nM Wnt-C59 or plus Wnt3a, (50, 100, 200 ng/ml) for 24 h, White arrowhead show actin on the tip of different neurites. B Graph shows quantification of average of neurites length (see left graph) and recovery of dendritic complexity (see right graph). The results are presented as the mean ± SEM for n = 5 experiments, **p < 0.01, ***p < 0.001. Bonferroni’s test. Bar represents 10 μm