Fig. 2
From: Morphological neurite changes induced by porcupine inhibition are rescued by Wnt ligands
A Porcupine inhibitor, Wnt-C59, produces a loss of contacts with other types of cells in culture. A Sister cultures of hippocampal neurons were used to quantify the contact of neurons with other cell types, without specifying the cell type; these contacts of neuronal processes decrease by half, depending also on the concentration of the inhibitor. See representative micrographs of control neurons and treated with 10 nM Wnt-C59 for 24 h. and zoom. B The figure shows a representative blot of some proteins of the Wnt pathway, under the inhibitory activity of Wnt-C59 in hippocampal neurons. Graph shows quantification of these contacts. The results are presented as the mean ± SEM for n = 10 experiments), *p < 0.05, **p < 0.01. Bonferroni’s test. Bar represents 10 μm