Fig. 1
From: Morphological neurite changes induced by porcupine inhibition are rescued by Wnt ligands
A Porcupine inhibitor, Wnt-C59 induces morphological change in dendritic arbor. Hippocampal neurons were cultured until 4DIV and treated with different concentration of Wnt-C59 (0.01, 0.1, 1, 10, 100 nM) for 36 h, and stained with MAP1B (red), ATF-2 (blue) and F-actin marker phalloidin (green). Neurons were imaged using a confocal microscope to evaluate neuronal morphology. A Representative Micrographs are showed, Wnt-C59 produces a decrease of the dendritic arbor in these embryonic neurons; Interestingly, a large amount of terminal filopodia/lamellipodia was observed with Wnt-C59 treatment (A, see white arrow). B The quantification of these processes is shows as representative data from embryonic cultured neurons. The results are presented as the mean ± SEM for n: 10–12 experiments), **p < 0.01. Bonferroni’s test. Bar represents 10 μm