Table 2 Primers for Real-time PCR
From: FZD5 prevents epithelial-mesenchymal transition in gastric cancer
Genes or binding sites | Primer (5′ to 3′) |
|---|---|
CDH1-forward | GCCATCGCTTACACCATCCTCAG |
CDH1-reverse | CTCTCTCGGTCCAGCCCAGTG |
ELF3-forward | ATGGTTTTCGTGACTGCAAGAA |
ELF3-reverse | CAGTACTCTTTGCTCAGCTTTC |
FZD5-forward | TCCTCTGCATGGATTACAACC |
FZD5-reverse | GACACTTGCACACGAACG |
GAPDH-forward | CAGGAGGCATTGCTGATGAT |
GAPDH-reverse | GAAGGCTGGGGCTCATTT |
LRP5-forward | CCCTACATCATTCGAGGAATGG |
LRP5-reverse | CCGAGTTCAAATCCAGGTAGTA |
VIM-forward | GGTGGACCAGCTAACCAACG |
VIM-reverse | TTGCAGGGTGTTTTCGGCTT |
ZEB1-forward | CAGGCAAAGTAAATATCCCTGC |
ZEB1-reverse | GGTAAAACTGGGGAGTTAGTCA |
SNAI2 BS1-forward | ATTTGGGCGGCTACTATGT |
SNAI2 BS1-reverse | CCTTACAACAATTCTGAGGC |
SNAI2 BS2-forward | ACCTGCCTCAGAATTGTTG |
SNAI2 BS2-reverse | GAACTCCTGACACCGTGAT |
TEAD1 BS1-forward | GTCCAGTCCCTATCCCTT |
TEAD1 BS1-reverse | AAATGCCTCTTTTCTATGTG |
TEAD1 BS2-forward | GGCAGGATAATCGCTTGA |
TEAD1 BS2-reverse | GGATACTTACTGAGCCCTTTAC |