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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: The eEF2 kinase-induced STAT3 inactivation inhibits lung cancer cell proliferation by phosphorylation of PKM2

Fig. 5

eEF2K phosphorylates PKM2. a An in vitro phosphorylation assay was performed with the indicated periods of time by incubation of 1 μg eEF2K, 8 μM ATP, and 1 μg of recombinant PKM2 or eEF2 in the presence or absence of CaM. Phosphorylation levels of substrates were detected by autoradiography. *: non-specific band. b An in vitro phosphorylation assay was performed with the indicated periods of time by incubation of 1 μg eEF2K, 8 μM ATP, CaM, and 1 μg of recombinant PKM2 in the presence or absence of 5 μM JAN-384 (upper panel). The relative phosphorylation levels of the PKM2 were quantified (lower panel). Data are presented as means ± S.E.M., n = 3, ** P < 0.01, ***P < 0.001. c An in vitro phosphorylation assay was performed by incubation of 8 μM ATP, CaM, and 1 μg of recombinant PKM2 in the presence of WT eEF2K, eEF2K D274A, or eEF2K K170M mutants

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Cell Communication and Signaling

ISSN: 1478-811X