Fig. 3

miR-941 inhibition upregulates Keap1, suppressing Nrf2 signaling in human endometrial cells. T-HESC cells were transduced with the pre-microRNA-941 anti-sense lentivirus (“antagomiR-941”), with selection by puromycin the stable cells were established, with control cells transduced with microRNA anti-sense control lentivirus (“antaC”); Expression of mature miR-941 and listed mRNAs were tested by qPCR assays (a, c, e and f); Keap1 3′-UTR activity was shown (b), with expression of listed proteins in total cell lysates (d) tested by Western blotting; The relative NQO1 activity was tested as well (g). The primary human endometrial cells were infected with antagomiR-941 or antaC, with expression of listed genes tested by qPCR (h, i, k, and l) and Western blotting (j) assays. The relative NQO1 activity was tested as well (m). Expression of listed protein was quantified and normalized (d and j). Data were presented as mean ± SD (n = 5), and results were normalized. * P < 0.05 vs. “antaC” cells. Experiments in this figure were repeated five times with similar results obtained