Fig. 6

CYP1A1 is involved in phagocytosis of bacteria in macrophages during sepsis. a-d CYP1A1 overexpression, CYP1A1 knockdown and WT PMs were treated with E. coli (MOIs = 30) for 40 min. WT PMs were pre-treated with Rhapontigenin (10 μM) or 12(S)-HETE (500 nM) for 2 h before E.coli onset. a Cells were washed and subjected to intracellular bacteria count detection. Group means were compared by Student’s t test (n = 6). b Cells were collected for SR-A mRNA measure. Results were compared by one-way ANOVA. Data are shown as mean ± SEM of three independent experiments. c Cells were lysed for western blotting analysis of SR-A protein expression. d SR-A protein expression was quantified by densitometry using GAPDH as the gel-loading control. Results were compared by one-way ANOVA. e CYP1A1 knockdown macrophages and Rhapontigenin-treated WT macrophages were pre-treated with SR-A antibody (3 μg/ml) for 12 h respectively and then cultured with E.coli for 40 min. Cells were washed and subjected to intracellular bacteria count detection. Group means were compared by Student’s t test (n = 6). *p < 0.05. NS, no statistical difference