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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Small molecule-induced simultaneous destabilization of β-catenin and RAS is an effective molecular strategy to suppress stemness of colorectal cancer cells

Fig. 5

Effects of KYA1797K on the tumorigenic potential and stemness of CRC PDTX. NOD/SCID mice bearing CRC patient tumors were treated with vehicle or KYA1797K (25 mg/kg) via i.p. injection for 28 days (N = 5). a Immunoblot analyses of vehicle or KYA1797K treated PDTX using indicated antibodies. b Volumes of subcutaneous tumors treated with vehicle or KYA1797K were measured using calipers two times a week for 28 days. c Final tumor volumes of vehicle- or KYA1797K-treated CRC PDTX. d Hematoxylin and eosin staining of vehicle- or KYA1797K-treated PDTX. Scale bar represents 200 μm. e IHC analyses of vehicle- or KYA1797K-treated PDTX using the indicated antibodies. Scale bar represents 100 μm. Mean intensities of fluorescence of indicated markers were quantified using Zen software in at least three different tissue samples. f qRT-PCR analyses of LGR5, CD44, CD133, and CD166 mRNAs in vehicle- or KYA1797K-treated CRC PDTX samples. Quantitative data are presented as mean ± standard deviation. * P < 0.05, ** P < 0.005, *** P < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X