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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Activation of cannabinoid receptor type 2-induced osteogenic differentiation involves autophagy induction and p62-mediated Nrf2 deactivation

Fig. 3

CNR2 agonists-induced osteogenic differentiation is blocked by autophagy inhibitor 3-MA. hFOB 1.19 cells were cultured at 34 °C until reaching confluence, and transferred to 39 °C. These cells were then treated with 2 mM 3-MA, 50 nM HU308 or 10 μM JWH133 for a-c 96 h or d 192 h. a The ALP activity was determined, and shown as mean ± standard deviation. The mRNA and protein levels of osteocalcin and osteopontin were determined with b real-time RT PCR and c western blotting, respectively. Data were shown as mean ± standard deviation. Cell mineralization was determined with Alizarin red staining. Heavier staining indicated stronger mineralization

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Cell Communication and Signaling

ISSN: 1478-811X