Fig. 2

Flow regulates YAP. a Flow-induced up regulation of YAP and pYAP at 24 and 48 h high shear flow. Actin given as loading control. Representative of 2 experiments for each time point. b Morphological characteristics of cells treated with siRNA. siCtl; (i and iii) or siYAP–treated ECs (ii and iv) under high shear flow imaged under bright field or for F-actin staining (red) Scale bar = 50 μm. Direction of flow as indicated. Representative of 3 independent experiments. Quantification of cellular alignment determined by counting the number of cells with actin stress fibers that were parallel to the direction of the major axis of cells. Approximately 1000 cells were counted, from 3 to 4 biological replicates. p < 0.05 by paired t-test. c Morphological characteristics of cells treated with siCtl or siTAZ. Two experiments p < 0.0001. Cell orientation angles with respect to the flow direction were used for the measurement of alignment when cells aligned to flow