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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Epigenetic induction of tumor stemness via the lipopolysaccharide-TET3-HOXB2 signaling axis in esophageal squamous cell carcinoma

Fig. 5

HOXB2 served as the downstream target of TET3 and participated in inducing the stemness of ESCC cells. a Dot-blot was performed to detect the 5hmC DNA level of ESCC cells with overexpression of TET3 (left panel). The right panel shows the membrane stained with methylene blue as an internal reference. b Heat map of the most up-regulated (red) and down-regulated (blue) genes (based on p value) in TET3-overexpression group compared with Control group analyzed with Nano-hmC-Seal-seq. c Scatterplot of p values for all genes in both groups analyzed with Nano-hmC-Seal-seq. Significantly up-regulated and down-regulated proteins in TET3-overexpression cells were highlighted in red and blue, respectively. d RT-qPCR and Western blot were performed to detected HOXB2 expression in ESCC cells with knockdown or overexpression of TET3. e RT-qPCR and Western blot were performed to detected HOXB2 expression in ESCC cells with PBS or LPS stimulation. f RT-qPCR was performed to detect stemness-related genes mRNA level in ESCC cells with knockdown of HOXB2 or/and overexpression of TET3. (ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001)

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Cell Communication and Signaling

ISSN: 1478-811X