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Table 1 Comparison of AblΔIDR rescue of adult and embryonic viability with other Abl constructs

From: Abelson kinase’s intrinsically disordered region plays essential roles in protein function and protein stability

Construct

Residues changed or deleted

Percent rescue of adult viability in abl4/Df(3L)st-j7 (normalized to WT)*

Probability that viability is higher than adults with no transgene

Probability that rescue is better or worse than that by Abl WT

Probability that rescue is better or worse than that by Abl ΔCR1

Percent rescue of progeny embryonic viabilty of abl4/Df(3L)st-j7 X abl4/+**

Probability that rescue is worse than that by Abl WT

Probability that rescue is better or worse than that by Abl ΔCR1

Percent rescue of progeny embryonic viabilty of abl4 maternal germ line clone X abl4/+***

Probability that rescue is worse than that of Abl WT

Probability that rescue is better or worse than that of ablMZ

Probability that rescue is better or worse than that by Abl ΔCR1

No Transgene

N/A

20.4 (n = 145)#

N/A

p < 0.0001

p = 0.4474

N/A

N/A

N/A

8.2% (n = 527)#

p < 0.0001

n/a

p = 0.0246

Abl WT

N/A

100% (n = 386)#

p < 0.0001

N/A

p ≤ 0.0001

89.4 (n = 901)#

N/A

p < 0.0001

38.9% (n = 540)#

n/a

p < 0.0001

p < 0.0001

Abl∆IDR

679–1398

48.1% (n = 223)

p = 0.0075

p < 0.0001

p = 0.3064

26.2% (n = 378)

p < 0.0001

p < 0.0001

1.1% (n = 569)

p < 0.0001

p < 0.0001

p < 0.0001

AblΔFABD

1418–1520

140% (n = 173)#

p < 0.0001

p < 0.0001

p < 0.0001

97.1 (n = 362)#

p < 0.0001

p < 0.0001

34.6% (n = 515)#

p = 0.16

p < 0.0001

p < 0.0001

AblKDΔFABD

K417N 1418–1520

81.5% (n = 585)#

p < 0.0001

p = 0.0108

p = 0.0029

52.1 (n = 259)#

p < 0.0001

p = 0.7268

28.2 (n = 560)#

p = 0.0002

p < 0.0001

p < 0.0001

AblΔCR1

734–789

30.7% (n = 48)#

p = 0.4474

p < 0.0001

N/A

53.9 (n = 267)#

p < 0.0001

N/A

12.4% (n = 606)#

p < 0.0001

p = 0.02

N/A

  1. Comparison of AblΔIDR rescue of adult and embryonic viability in Abl null mutant background with other Abl constructs with statistical significance. A two-tailed Fisher's Exact Test was used to determine p-values of whether there was a statistically significant difference in extent of rescue between genotypes (see Methods)
  2. #Data from Rogers et al. 2016 for comparison; n = number of adults/embryos scored; N/A = not applicable; italics = rescue better than indicated genotype; bold italics = rescue worse than indicated genotype
  3. *Graphed in Fig. 1d
  4. **Graphed in Fig. 1e
  5. ***Graphed in Fig. 1f
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Cell Communication and Signaling

ISSN: 1478-811X