Table 2 Antibodies used for immunocytochemistry
From: Chronic inflammation of middle ear cholesteatoma promotes its recurrence via a paracrine mechanism
| Â | Species | Number | Manufacturer | Dilution | Incubation |
|---|---|---|---|---|---|
Primary antibody | |||||
Cytokeratin 14 | Rabbit | PA5-28,002 | Invitrogen | 1:200 | 4 °C, o/n |
Cytokeratin 16 | Mouse | sc-377224 | Santa Cruz | 1:100 | 4 °C, o/n |
Cytokeratin 18 | Mouse | SC-32329 | Santa Cruz | 1:500 | 4 °C, o/n |
Cytokeratin 19 | Rabbit | NBP1-78,278 | Novus Biologicals | 1:100 | 4 °C, o/n |
Secondary antibody | |||||
Anti-rabbit Alexa 488 | Goat | A32731 | Invitrogen Molecular Probes | 1:300 | RT, 1Â h |
Anti-mouse Alexa 555 | Goat | A21422 | Invitrogen Molecular Probes | 1:300 | RT, 1Â h |