Fig. 3

HDAC3 inhibition activates Nrf2 signaling in vitro. a and c Expression of total (T-Nrf2), cytosolic (c-Nrf2) and nuclear (n-Nrf2) protein levels of Nrf2 were detected by Western blot. b, d and e The quantitative analysis of each immunoblots. f The mRNA level and the quantification of Keap1 were evaluated by qRT-PCR. g the protein level of Keap1 were detected by western blot. h The quantitative analysis of (g). i The mRNA level and the quantification of the Nrf2 target gene were evaluated by qRT-PCR. j The mRNA level and the quantification of the NF-κB target gene were evaluated by qRT-PCR. The data are represented as the means ± SEM (n = 4). Significance: *P < 0.05 vs. Con or MAN in scrambled HUVECs; #P < 0.05 vs. HG-PA in scrambled HUVECs. All above results in graphs were normalized to the first group